human mesothelial met 5a cells (ATCC)
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human mesothelial met 5a cells
Human Mesothelial Met 5a Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 684 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+met+5a+mesothelial+cells/MeT-5A/pmc13208172-49-6-14
Average 96 stars, based on 684 article reviews
Human Mesothelial Met 5a Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 684 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+met+5a+mesothelial+cells/MeT-5A/pmc13208172-49-6-14
Average 96 stars, based on 684 article reviews
human mesothelial met 5a cells - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "Quercetin–Arctigenin Co-Treatment Induces Mitochondrial Dysfunction and Apoptotic Cell Death Through Metabolic Stress in Malignant Mesothelioma Cells"
Article Title: Quercetin–Arctigenin Co-Treatment Induces Mitochondrial Dysfunction and Apoptotic Cell Death Through Metabolic Stress in Malignant Mesothelioma Cells
Journal: Life
doi: 10.3390/life16050774
Figure Legend Snippet: Effects of quercetin and arctigenin on relative metabolic activity in mesothelioma and mesothelial cells. ( A – C ) MSTO-211H and MeT-5A cells were treated with increasing concentrations of QUE or ATG for ( A ) 24 h, ( B ) 48 h, and ( C ) 72 h. Relative metabolic activity was measured using the MTT assay. Data are presented as the mean ± SD from at least three independent experiments. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05 vs. control).
Techniques Used: Activity Assay, MTT Assay, Control
Figure Legend Snippet: Combined treatment with quercetin and arctigenin reduces metabolic activity and intracellular ATP production in mesothelioma cells. ( A – C ) MSTO-211H and MeT-5A cells were treated with QUE (40 μM), ATG (50 μM), or their combination for ( A ) 24 h, ( B ) 48 h, and ( C ) 72 h. Relative metabolic activity was measured using the MTT assay. ( D – F ) Intracellular ATP levels were measured after ( D ) 24 h, ( E ) 48 h, and ( F ) 72 h treatment. Data are presented as the mean ± SD from at least three independent experiments. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05, *** p < 0.001, **** p < 0.0001 vs. control).
Techniques Used: Activity Assay, MTT Assay, Control
Figure Legend Snippet: Combination treatment suggests a potential synergistic interaction, reduces cell growth capacity, and increases caspase activity in mesothelioma cells. ( A ) Combination index (CI) values were calculated for QUE (40 μM) and ATG (50 μM) co-treatment in MeT-5A and MSTO-211H cells at 24, 48, and 72 h. CI values < 1 may be indicative of a potential synergistic interaction under the experimental conditions tested. ( B ) Cell growth capacity was assessed by crystal violet staining following 48 h treatment with QUE, ATG, or their combination. ( C ) Caspase 3/7 activity was measured after 48 h of treatment to evaluate apoptosis-associated protease activation. Data are presented as the mean ± SD from at least three independent experiments. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control).
Techniques Used: Activity Assay, Staining, Activation Assay, Control
Figure Legend Snippet: Combined treatment with quercetin and arctigenin suggests increased apoptotic cell populations in mesothelioma cells. Representative Annexin V dot plots showing apoptotic cell populations in MeT-5A and MSTO-211H cells following treatment with QUE (40 μM), ATG (50 μM), or their combination for 48 h. Dot plots represent cells stained with Annexin V and PI, where color intensity indicates cell density and quadrants correspond to live, early apoptotic, and late apoptotic cell populations. Percentages of live, early apoptotic, and late apoptotic cells are indicated. Lower panels show quantitative analysis of live cells (%) and total apoptotic cells (%). Data are presented as the mean ± SD from at least three independent experiments. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (** p < 0.01, **** p < 0.0001 vs. control).
Techniques Used: Staining, Control
Figure Legend Snippet: Co-treatment with quercetin and arctigenin is associated with modulation of mitochondrial apoptosis-associated signaling and energy stress-related protein expression in mesothelioma cells. ( A ) Representative Western blot analysis of apoptosis-associated proteins in MeT-5A and MSTO-211H cells treated with quercetin (QUE, 40 μM), arctigenin (ATG, 50 μM), or their combination for 48 h. Expression levels of anti-apoptotic proteins Mcl-1, Bcl-xL, and Bcl-2, as well as apoptosis execution markers cleaved caspase-3 and cleaved PARP, were examined. β-actin was used as the loading control. ( B ) Representative Western blot analysis of energy stress- and mitochondrial protein expression-related markers. Expression levels of phosphorylated AMPK (p-AMPK), total AMPK, and selected oxidative phosphorylation (OXPHOS) complex subunits (ATP5A, UQCRC2, SDHB, COX II, and NDUFB8) were evaluated in MeT-5A and MSTO-211H cells following treatment with QUE, ATG, or their combination for 48 h. Relative protein expression levels were normalized to β-actin, and the p-AMPK/AMPK ratio was calculated to evaluate AMPK activation. Densitometric values are presented as mean ± SD from three independent experiments (n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control). Faint background signals may be present due to exposure sonditions and do not affect the interpretation of the results.
Techniques Used: Expressing, Western Blot, Control, Phospho-proteomics, Activation Assay
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